| Gene | Rab3il1 |
| Chromosome | 19 |
| Deletion Start | 10,018,352 |
| Deletion End | 10,034,569 |
| Deletion Size | 16,218 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,018,882 | 10,018,903 | CCCTCATCCTCACTTCCATCTG |
| TUP | 10,018,905 | 10,018,929 | TAGGGCCATCGGTTCTTGAAGCTGA |
| TUR | 10,018,960 | 10,018,978 | GTGAACAGGGAGCAAGGTC |
| TDF | 10,033,715 | 10,033,734 | GGGTCACACCCTCTCCATTC |
| TDP | 10,033,735 | 10,033,758 | TGCCCACAGATCACCGCAGTATGC |
| TDR | 10,033,789 | 10,033,808 | TCCTGCCGAACCAGACCTTG |
PCR Primers
| Primer | Sequence |
| SU | CCGAGAGCCATTGTAGGTTC |
| SD | TTGGAGACATTTGCCCTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12658A-D5 | VGB6 | C57BL/6NTac | |
| 12658A-H8 | VGB6 | C57BL/6NTac | |
| 12658A-E9 | VGB6 | C57BL/6NTac | |
| 12658A-B2 | VGB6 | C57BL/6NTac | |
