| Gene | Spp2 |
| Chromosome | 1 |
| Deletion Start | 88,407,153 |
| Deletion End | 88,420,726 |
| Deletion Size | 13,574 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,407,838 | 88,407,858 | CCATGAAACCCACCCACTCTG |
| TUP | 88,407,860 | 88,407,887 | TGGACTTGATAGTGCTGTTTCCCGATCA |
| TUR | 88,407,908 | 88,407,928 | CAGCAAGCCCGAGATAATGTC |
| TDF | 88,420,505 | 88,420,523 | AGCGAGGGCTCAGGTTGTG |
| TDP | 88,420,526 | 88,420,553 | TGCCTTGGTCTCATCCATTTATGCCTGA |
| TDR | 88,420,558 | 88,420,578 | GGATGGAAGGGAGAAGCAGAC |
PCR Primers
| Primer | Sequence |
| SU | TTGAGAGGTGGAGGGTGAAG |
| SD | GAGGGAAGAAAGAACTGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12659C-A1 | VGB6 | C57BL/6NTac | |
| 12659C-H5 | VGB6 | C57BL/6NTac | |
| 12659C-D1 | VGB6 | C57BL/6NTac | |
