| Gene | Jakmip1 |
| Chromosome | 5 |
| Deletion Start | 37,085,373 |
| Deletion End | 37,107,315 |
| Deletion Size | 21,943 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,086,164 | 37,086,184 | TCGGAAGCTGAGAGCAGTTAG |
| TUP | 37,086,187 | 37,086,214 | TGAGCCAGTGTTCAGCTTGCTTTCTTCA |
| TUR | 37,086,223 | 37,086,243 | GCTGCACAGCAATGGAGAATG |
| TDF | 37,106,534 | 37,106,553 | GACCAGTCTGCCAGGATCTG |
| TDP | 37,106,559 | 37,106,581 | ATCCAGGCCCAGCTTTGTCCTGC |
| TDR | 37,106,593 | 37,106,613 | TCTCCCTTTCAGCACACTCTC |
PCR Primers
| Primer | Sequence |
| SU | AGGAGGTGAGACTCTGCCTG |
| SD | AATGAGGCTGAGTGTCCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12661A-D9 | VGB6 | C57BL/6NTac | |
| 12661A-F7 | VGB6 | C57BL/6NTac | |
