| Gene | Il33 |
| Chromosome | 19 |
| Deletion Start | 29,949,674 |
| Deletion End | 29,959,116 |
| Deletion Size | 9,443 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,950,527 | 29,950,549 | CGTTGCCTGAATTTGCATTTGAC |
| TUP | 29,950,550 | 29,950,570 | TGCTCCCTCAGATGGCAGTGC |
| TUR | 29,950,624 | 29,950,644 | AGCAGGAGGGTTGCGAGTTTC |
| TDF | 29,958,128 | 29,958,148 | TGAGTTGGCTCCCCAGTATTC |
| TDP | 29,958,150 | 29,958,171 | TTGGCAGGCGACTCACAACACC |
| TDR | 29,958,186 | 29,958,206 | CAGAGGCACCTGACTGTCTTG |
PCR Primers
| Primer | Sequence |
| SU | AACAGTACTGAGATTTCAACAC |
| SD | CAAACCATGCACTGTGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12663E-A11 | VGB6 | C57BL/6NTac | |
| 12663E-H2 | VGB6 | C57BL/6NTac | |
