| Gene | 9530003J23Rik |
| Chromosome | 10 |
| Deletion Start | 117,238,577 |
| Deletion End | 117,234,357 |
| Deletion Size | 4,221 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,238,524 | 117,238,543 | TCCTGCTCTCCATCACCATC |
| TUP | 117,238,498 | 117,238,523 | CAGGGCAAGGTCTACGACCGATGTTC |
| TUR | 117,238,457 | 117,238,477 | GGAAACCAGCTAGACCAAGAC |
| TDF | 117,234,831 | 117,234,851 | TCTGTCCTGTGCTCTACTGAG |
| TDP | 117,234,807 | 117,234,828 | TGTCAGGAGGCGCAAGGCTCTT |
| TDR | 117,234,770 | 117,234,793 | CAGGTACATCCAGAAAGCAGTAAC |
PCR Primers
| Primer | Sequence |
| SU | CTAGCTCCCGGTTCTTTGTG |
| SD | TGACGAAGGCCTCAGATACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12664A-A2 | VGB6 | C57BL/6NTac | |
| 12664A-E3 | VGB6 | C57BL/6NTac | |
| 12664A-G2 | VGB6 | C57BL/6NTac | |
| 12664A-G6 | VGB6 | C57BL/6NTac | |
