| Gene | C430048L16Rik |
| Chromosome | 4 |
| Deletion Start | 12,093,146 |
| Deletion End | 12,095,672 |
| Deletion Size | 2,527 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,094,575 | 12,094,598 | AGGAACAAATGCAGGAGTTTGGTA |
| TUP | 12,094,604 | 12,094,632 | TCTTCCTGGTTGTCTAATGAGAGGACACA |
| TUR | 12,094,651 | 12,094,672 | ACAGGAACAGTCATCTCCATCA |
| TDF | 12,095,359 | 12,095,383 | TCATGACGGAAAATTTGTTCCTGAC |
| TDP | 12,095,385 | 12,095,406 | TAAAGTTTAATTGTGGTTCAGG |
| TDR | 12,095,431 | 12,095,454 | GCATTGGCTTTGAATGGAAGATTC |
PCR Primers
| Primer | Sequence |
| SU | TTGTGAAGTAGCATTTGGCAG |
| SD | GCCTTCATTTCTTTATATGGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12665A-A8 | VGB6 | C57BL/6NTac | |
| 12665A-D10 | VGB6 | C57BL/6NTac | |
| 12665A-B12 | VGB6 | C57BL/6NTac | |
| 12665A-D6 | VGB6 | C57BL/6NTac | |
