| Gene | Efcab6 |
| Chromosome | 15 |
| Deletion Start | 84,044,310 |
| Deletion End | 84,018,631 |
| Deletion Size | 25,680 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,044,132 | 84,044,154 | ACAACACTGTCTACAGATACCAC |
| TUP | 84,044,110 | 84,044,131 | TCACTGTCTGCCCGCTGTGAGT |
| TUR | 84,044,068 | 84,044,089 | TCAGAAGGGTATGGGAGTCAAC |
| TDF | 84,019,605 | 84,019,626 | TTGACAGGGATTGAACTGACTC |
| TDP | 84,019,580 | 84,019,604 | TGTAGGTCACTTTCAGGAGTGCAGC |
| TDR | 84,019,531 | 84,019,550 | GAAGGACCTCCCATGCACAC |
PCR Primers
| Primer | Sequence |
| SU | AAAGCAGGTTGAAGGAATGG |
| SD | CAAATCTGGCATTAAAGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12666C-B3 | VGB6 | C57BL/6NTac | |
| 12666C-E6 | VGB6 | C57BL/6NTac | |
