| Gene | 3110062M04Rik |
| Chromosome | 6 |
| Deletion Start | 34,876,681 |
| Deletion End | 34,874,364 |
| Deletion Size | 2,318 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,876,097 | 34,876,114 | AGGCTTTCAGGGCCACAC |
| TUP | 34,876,069 | 34,876,095 | TGCTGGCTCTTTCCTGTTCCTTACGTA |
| TUR | 34,876,021 | 34,876,039 | CAGGCCAGAGCCCAGTTTG |
| TDF | 34,874,770 | 34,874,789 | CGGTTGGCTCAGGGTACATC |
| TDP | 34,874,742 | 34,874,767 | TAGCACTGTCAAACTCGTGCCTTGGA |
| TDR | 34,874,715 | 34,874,732 | GCTGTCTCGGCACAAAGG |
PCR Primers
| Primer | Sequence |
| SU | CCTTTCTTCCTTCCTTTGGG |
| SD | CCTAGACTGGCAGGGAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12667A-E11 | VGB6 | C57BL/6NTac | |
| 12667B-C3 | VGB6 | C57BL/6NTac | |
| 12667B-E3 | VGB6 | C57BL/6NTac | |
| 12667B-F4 | VGB6 | C57BL/6NTac | |
| 12667B-H5 | VGB6 | C57BL/6NTac | |
