| Gene | Ctdp1 |
| Chromosome | 18 |
| Deletion Start | 80,469,543 |
| Deletion End | 80,445,891 |
| Deletion Size | 23,653 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,468,769 | 80,468,789 | AGCCCAGACTATCGGGTGATG |
| TUP | 80,468,741 | 80,468,767 | ACCTGGTCCTGGATTTCTTGTGAGGAC |
| TUR | 80,468,682 | 80,468,702 | GGCAGACCAAACCAAGTCAAG |
| TDF | 80,446,033 | 80,446,052 | CCAACAGCCTTGTTCCATCC |
| TDP | 80,446,006 | 80,446,031 | ACACCCATCCATTCCAAGGCCCATCC |
| TDR | 80,445,962 | 80,445,983 | TCAGCTTGCCTGTGTTAGAGTC |
PCR Primers
| Primer | Sequence |
| SU | TGTAGTCTCGATGCGTCAGG |
| SD | CTGCTGAGGACAGACCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12669A-F12 | VGB6 | C57BL/6NTac | |
| 12669A-E12 | VGB6 | C57BL/6NTac | |
| 12669A-G6 | VGB6 | C57BL/6NTac | |
| 12669A-C5 | VGB6 | C57BL/6NTac | |
| 12669A-G8 | VGB6 | C57BL/6NTac | |
