| Gene | Cript |
| Chromosome | 17 |
| Deletion Start | 87,025,663 |
| Deletion End | 87,035,095 |
| Deletion Size | 9,433 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,025,799 | 87,025,819 | TTGCCTGTCCCGTAAGCATAG |
| TUP | 87,025,820 | 87,025,844 | CCTTCCGCTCTGTGTTGAGAATGGC |
| TUR | 87,025,869 | 87,025,888 | GCGAGGACGACAACAGAAAC |
| TDF | 87,034,738 | 87,034,756 | GGGCTAGAAGCCAGCAAAC |
| TDP | 87,034,759 | 87,034,782 | AGGTTCCTCCTGTCTGCACCCCAC |
| TDR | 87,034,807 | 87,034,827 | GTCCAGCATCCACACTCTCAC |
PCR Primers
| Primer | Sequence |
| SU | CTTACCTAACTCTCCCGCCC |
| SD | TGAGAAATCTGAGGTCTGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12670B-G6 | VGB6 | C57BL/6NTac | |
| 12670B-F9 | VGB6 | C57BL/6NTac | |
