| Gene | Rhoj |
| Chromosome | 12 |
| Deletion Start | 75,308,827 |
| Deletion End | 75,308,997 |
| Deletion Size | 171 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGCAGAGAGAGGACCGACAG |
| TUP | | | AGCTGCGGCTGCAATGGACATGA |
| TUR | | | GTCGTTGGCGTAGCTCATCAG |
| TDF | | | CCTGAAGTGCGTGGTGGTC |
| TDP | | | AAGACCTGCTTGCTGATGAGCTACGCC |
| TDR | | | CAAACACAGTGGGCACGTATTC |
PCR Primers
| Primer | Sequence |
| SU | CTGCTGATATTTCTAGACTGC |
| SD | GTTGAGAAACATTTCTGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12672A-H6 | VGB6 | C57BL/6NTac | |
| 12672A-B7 | VGB6 | C57BL/6NTac | |
| 12672A-F5 | VGB6 | C57BL/6NTac | |
| 12672A-G10 | VGB6 | C57BL/6NTac | |
