| Gene | Zfp956 |
| Chromosome | 6 |
| Deletion Start | 47,963,363 |
| Deletion End | 47,964,514 |
| Deletion Size | 1,152 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,963,627 | 47,963,646 | ACACAGCAGGTCACTCTCAC |
| TUP | 47,963,647 | 47,963,669 | CAGGAATCGCCGTGCACCCACAG |
| TUR | 47,963,688 | 47,963,707 | GCTCTTGCCACATTGGACAC |
| TDF | 47,964,212 | 47,964,234 | TCACCAAGTCATGTGTCACCTAC |
| TDP | 47,964,238 | 47,964,260 | TCTGTTTAAAGGCGCAGCCAGGG |
| TDR | 47,964,265 | 47,964,284 | CAGGAGGGTCACTGGAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCTTGTCAGAGAGCAGCATC |
| SD | GCCCTAGAGAAATCTTGGAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12677A-B7 | VGB6 | C57BL/6NTac | |
| 12677A-D4 | VGB6 | C57BL/6NTac | |
| 12677A-D11 | VGB6 | C57BL/6NTac | |
| 12677A-E3 | VGB6 | C57BL/6NTac | |
| 12677A-F4 | VGB6 | C57BL/6NTac | |
| 12677A-G10 | VGB6 | C57BL/6NTac | |
