| Gene | Ces2a |
| Chromosome | 8 |
| Deletion Start | 104,734,075 |
| Deletion End | 104,741,423 |
| Deletion Size | 7,349 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,734,258 | 104,734,280 | GTGGAGAATGACAGGTGACAGTG |
| TUP | 104,734,292 | 104,734,316 | TGCTTTGTAATGTCCCACCAGGGCA |
| TUR | 104,734,365 | 104,734,385 | TTCTGTCCCGGTTGTCTCCTC |
| TDF | 104,741,209 | 104,741,227 | TGCTGGCTGGCTTATCCAC |
| TDP | 104,741,234 | 104,741,257 | CCCAACAGTGAGGGTCTACCCTCC |
| TDR | 104,701,833 | 104,701,854 | ACTGCTCATCATGGTCCAACAC |
PCR Primers
| Primer | Sequence |
| SU | CCTACATTGGGATGGTGAGG |
| SD | TGAAGCACCCACTTCATTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 39395 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12678A-H10 | VGB6 | C57BL/6NTac | |
| 12678B-H7 | VGB6 | C57BL/6NTac | |
| 12678B-H1 | VGB6 | C57BL/6NTac | |
