| Gene | Zdhhc7 |
| Chromosome | 8 |
| Deletion Start | 120,088,065 |
| Deletion End | 120,082,538 |
| Deletion Size | 5,528 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,087,977 | 120,087,998 | ACTCAGCATCCTCTTCGTCCTC |
| TUP | 120,087,942 | 120,087,968 | ACATGGCAGACAGGGTGTGGTTCATCC |
| TUR | 120,087,923 | 120,087,941 | ACCATGCCACAGCCATCTC |
| TDF | 120,082,746 | 120,082,767 | GGCTGACTGTGGATCTCTTCTC |
| TDP | 120,082,720 | 120,082,744 | TTGCTGCTCTGGCTTCTTCTGCTCC |
| TDR | 120,082,684 | 120,082,702 | CGTGGGCTTCTCGCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTTGGCTAGTTTGGTGCTT |
| SD | TTCAGTGCTGGGTGTCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12679A-A5 | VGB6 | C57BL/6NTac | |
| 12679A-E1 | VGB6 | C57BL/6NTac | |
| 12679A-E2 | VGB6 | C57BL/6NTac | |
| 12679A-F9 | VGB6 | C57BL/6NTac | |
| 12679A-G9 | VGB6 | C57BL/6NTac | |
