12682   Lrrc14b     Status: ES cell colonies screened / QC positives

GeneLrrc14b
Chromosome13
Deletion Start74,363,956
Deletion End74,360,685
Deletion Size3,272
Genome Build38
MGI IDMGI:2145269
NCBI Gene ID432779
BAC ID460I6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,363,86074,363,881GCCTACAACCTCTACCCACTTC
TUP74,363,83274,363,856TCAAGGCCAGTTACCTGCTAGAGCA
TUR74,363,80974,363,827GCAGAGCACGGGTTACATC
TDF74,361,24374,361,266CACCTGTACCCTTCTACCTTCTTC
TDP74,361,21774,361,239CTGCTGAGCCAGGCTGCCCAAAC
TDR74,361,17774,361,197GTCTGTGATGTTGCACTCCTC

PCR Primers

PrimerSequence
SUTAGGCTACTTGTGCAAGTATC
SDCCTCACAGCATCAGAGCTAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR73 bp++-
TDFTDR90 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12682B-C3VGB6C57BL/6NTac
12682B-E7VGB6C57BL/6NTac
12682B-E4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image