| Gene | Csdc2 |
| Chromosome | 15 |
| Deletion Start | 81,946,757 |
| Deletion End | 81,949,340 |
| Deletion Size | 2,584 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,947,674 | 81,947,695 | AGTGTGGGATGTGGAACGAGAG |
| TUP | 81,947,697 | 81,947,720 | TAACCTTCAAGGCAGCCTGGTGGA |
| TUR | 81,947,721 | 81,947,740 | AGAGGCCCAAGGAGCCAGAG |
| TDF | 81,948,607 | 81,948,627 | GGGCGTCTGTAAGCAGTTCTC |
| TDP | 81,948,631 | 81,948,650 | CTCCCAGGGCCACGGCTTCA |
| TDR | 81,948,659 | 81,948,679 | GATGTCCTCGGAGCCATTCTC |
PCR Primers
| Primer | Sequence |
| SU | AGCTCCGCAAACAAACATTC |
| SD | ATAACCCAGGTCCAGCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12685A-C8 | VGB6 | C57BL/6NTac | |
| 12685A-D9 | VGB6 | C57BL/6NTac | |
| 12685A-F2 | VGB6 | C57BL/6NTac | |
| 12685A-G8 | VGB6 | C57BL/6NTac | |
| 12685A-H3 | VGB6 | C57BL/6NTac | |
| 12685A-H12 | VGB6 | C57BL/6NTac | |
