| Gene | Clic6 |
| Chromosome | 16 |
| Deletion Start | 92,498,457 |
| Deletion End | 92,528,165 |
| Deletion Size | 29,709 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,499,377 | 92,499,396 | GGAGGCCAAATCCACCGAAC |
| TUP | 92,499,400 | 92,499,423 | AGGAGGAATCCCAGGCAGAGCTCA |
| TUR | 92,499,429 | 92,499,447 | TGGGCTCCTCAGCTAAGTG |
| TDF | 92,527,371 | 92,527,390 | CGCCTTCATCCTTTGCTGTG |
| TDP | 92,527,391 | 92,527,416 | TCTGACTATGCCCAAACCTGCACCAG |
| TDR | 92,527,423 | 92,527,442 | CGGAAGCCTGTCCTACACTC |
PCR Primers
| Primer | Sequence |
| SU | GGCTGTCATTGGCTAGCTTC |
| SD | ATGATCGGTGGCTGCCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12686A-A12 | VGB6 | C57BL/6NTac | |
| 12686A-B12 | VGB6 | C57BL/6NTac | |
| 12686A-C9 | VGB6 | C57BL/6NTac | |
| 12686A-G1 | VGB6 | C57BL/6NTac | |
