| Gene | AU023871 |
| Chromosome | 17 |
| Deletion Start | 35,066,167 |
| Deletion End | 35,064,316 |
| Deletion Size | 1,852 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,065,602 | 35,065,623 | CGCACAGTGCTTCAAGTGTTAG |
| TUP | 35,065,579 | 35,065,598 | ACAAGGCAGGTTGCCGGCCT |
| TUR | 35,065,544 | 35,065,562 | CATCTGGGCCTCGACCTAC |
| TDF | 35,064,873 | 35,064,895 | AACTGCACTCAATTTCCCTATCC |
| TDP | 35,064,849 | 35,064,871 | ACCCGCACAATCCCTAACCCTGA |
| TDR | 35,064,825 | 35,064,845 | TCGCAAGGACTCAGACTCAAG |
PCR Primers
| Primer | Sequence |
| SU | CCATGGTAGTTGTTCGCAA |
| SD | AGCAGCCAATGAGCAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12687A-A5 | VGB6 | C57BL/6NTac | |
| 12687A-B1 | VGB6 | C57BL/6NTac | |
| 12687A-C5 | VGB6 | C57BL/6NTac | |
| 12687A-A10 | VGB6 | C57BL/6NTac | |
