| Gene | Mro |
| Chromosome | 18 |
| Deletion Start | 73,869,961 |
| Deletion End | 73,877,946 |
| Deletion Size | 7,986 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,870,058 | 73,870,079 | ACGTCCCGTCCCACTATGTAAG |
| TUP | 73,870,081 | 73,870,110 | TGGGAGAGCCTGCTATCTCTGTAGTTAGCG |
| TUR | 73,870,114 | 73,870,133 | CAGAAGGAACCCAGGCAGAG |
| TDF | 73,877,449 | 73,877,468 | GAGCCTGTGTCCCGTATCTG |
| TDP | 73,877,469 | 73,877,494 | AAACCCAGAAAGGTGCCGAGTTTCCA |
| TDR | 73,877,502 | 73,877,523 | GCCTGTGGTTCTTCTGATCTTC |
PCR Primers
| Primer | Sequence |
| SU | AGAAGCACCGAACCTCACAC |
| SD | GACCTCCACAACCATGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12691A-F10 | VGB6 | C57BL/6NTac | |
| 12691A-C4 | VGB6 | C57BL/6NTac | |
