| Gene | Syt12 |
| Chromosome | 19 |
| Deletion Start | 4,467,937 |
| Deletion End | 4,447,634 |
| Deletion Size | 20,304 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,467,356 | 4,467,379 | GTCATCCTTCATTGCTCTTCCATC |
| TUP | 4,467,327 | 4,467,353 | ATTTGTTGAAGCAGGCGGTGTCTTTCA |
| TUR | 4,467,285 | 4,467,308 | CTGGTTATTGAGAGGAGCCGATTC |
| TDF | 4,447,960 | 4,447,981 | CCCAAGCCAGATGGGAAGATTC |
| TDP | 4,447,932 | 4,447,956 | TAGCTGTATGCACCTCACAGGGCCT |
| TDR | 4,447,892 | 4,447,913 | CAGGAGCAGCTAAAGGGTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GGAAGGAGGGATACAGTAGA |
| SD | TGCCACCACAGGAACTATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12694A-B8 | VGB6 | C57BL/6NTac | |
| 12694A-C9 | VGB6 | C57BL/6NTac | |
| 12694A-D2 | VGB6 | C57BL/6NTac | |
| 12694A-A7 | VGB6 | C57BL/6NTac | |
