| Gene | Slc22a26 |
| Chromosome | 19 |
| Deletion Start | 7,802,446 |
| Deletion End | 7,782,109 |
| Deletion Size | 20,338 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,801,863 | 7,801,888 | CCAGTTATTTCATTCAAGAGATGACC |
| TUP | 7,801,823 | 7,801,845 | TCAGAAATTTGACAACAGGACCA |
| TUR | 7,801,780 | 7,801,801 | GGTAAGTCCTTTGGGTGGGATA |
| TDF | 7,782,688 | 7,782,712 | AGCACTCCCATTATCTCTTTCCAAG |
| TDP | 7,782,662 | 7,782,686 | TGTCAGACAAGTAGGCTTTCGCCCA |
| TDR | 7,782,626 | 7,782,649 | ACAGGGAAGACTTCTGTTCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | GGTTCACAAAGGTTCTCAGC |
| SD | CATTGTTCAGCCACCACATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12703B-B8 | VGB6 | C57BL/6NTac | |
| 12703B-F2 | VGB6 | C57BL/6NTac | |
| 12703B-C4 | VGB6 | C57BL/6NTac | |
