| Gene | Moxd2 |
| Chromosome | 6 |
| Deletion Start | 40,887,491 |
| Deletion End | 40,878,750 |
| Deletion Size | 8,742 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,886,849 | 40,886,868 | AATCTCTGCCTCCAATGTCC |
| TUP | 40,886,818 | 40,886,846 | TCCACCCACTCAGCTATACTTCAGTTTGA |
| TUR | 40,886,789 | 40,886,811 | TTGGCTGCCGTTGTTCTTATTAG |
| TDF | 40,879,335 | 40,879,356 | GGAGTCCCAGCAGACAGTATTG |
| TDP | 40,879,305 | 40,879,333 | TCAAGACCATTGATGTGCGTGTCACAGTA |
| TDR | 40,879,272 | 40,879,289 | CAGTGCCATCCCTCAACC |
PCR Primers
| Primer | Sequence |
| SU | ACTTTACAGGTTTGCACCAG |
| SD | AGTAATGCCTTCTCCAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12705A-C5 | VGB6 | C57BL/6NTac | |
| 12705A-E3 | VGB6 | C57BL/6NTac | |
| 12705A-G10 | VGB6 | C57BL/6NTac | |
