| Gene | 9930021D14Rik |
| Chromosome | 17 |
| Deletion Start | 24,474,224 |
| Deletion End | 24,475,360 |
| Deletion Size | 1,137 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,474,452 | 24,474,471 | CTGGCCCAACCAAACTACTC |
| TUP | 24,474,478 | 24,474,501 | ATGTGGCCCAGAACATGGCAACCA |
| TUR | 24,474,538 | 24,474,556 | TGCCCGTCAAACAGCACAG |
| TDF | 24,475,103 | 24,475,121 | TTGTGCAGACACTCCCATC |
| TDP | 24,475,123 | 24,475,146 | ACTGGACCCAATGGGCAGAAGGTA |
| TDR | 24,475,153 | 24,475,174 | AGTCCTCTTCACCCAACCTATC |
PCR Primers
| Primer | Sequence |
| SU | ATCAGTGCATTGTCAGCAGG |
| SD | CATGCCCAGTTTGTTGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12710A-F10 | VGB6 | C57BL/6NTac | |
