| Gene | Itgbl1 |
| Chromosome | 14 |
| Deletion Start | 123,660,312 |
| Deletion End | 123,661,502 |
| Deletion Size | 1,191 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,660,330 | 123,660,352 | AACTTCTTGTTGCTGGTGTCCTC |
| TUP | 123,660,356 | 123,660,379 | TCTCTTCATTGGGCTGTCAGCTGC |
| TUR | 123,660,396 | 123,660,419 | AAACCAAACTTACCTCAGAGATGG |
| TDF | 123,661,171 | 123,661,190 | GCTTGGCCCACAGAGAAATG |
| TDP | 123,661,194 | 123,661,215 | CTGCAGGCCTCTTCTCCACCGA |
| TDR | 123,661,243 | 123,661,262 | GGCCACAGTCAGACACAGAC |
PCR Primers
| Primer | Sequence |
| SU | AACCATCTGCTTGTACAACTG |
| SD | ATGATTAGGAGCCACAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12718A-B8 | VGB6 | C57BL/6NTac | |
| 12718A-G12 | VGB6 | C57BL/6NTac | |
| 12718A-F10 | VGB6 | C57BL/6NTac | |
