| Gene | Msrb3 |
| Chromosome | 10 |
| Deletion Start | 120,871,107 |
| Deletion End | 120,849,985 |
| Deletion Size | 21,123 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,870,986 | 120,871,004 | CGGGCAGAGTGGGTATTTC |
| TUP | 120,870,958 | 120,870,983 | CTGCAGGAAATGGTACACCAGCATGC |
| TUR | 120,870,884 | 120,870,905 | GAGGACGGTGAGGGTTTGTAAC |
| TDF | 120,850,829 | 120,850,850 | CCCCACTGTTCACTGAGTTTAC |
| TDP | 120,850,798 | 120,850,824 | CAATGAAGCTCTCTCACAAGGTGTGCC |
| TDR | 120,850,732 | 120,850,750 | CAACATTGGCGGGCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GTGTCCTTGGATCTTCGAGC |
| SD | ATACGCTCTCTGTCACCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12720A-D4 | VGB6 | C57BL/6NTac | |
| 12720A-B3 | VGB6 | C57BL/6NTac | |
| 12720A-B7 | VGB6 | C57BL/6NTac | |
| 12720A-A11 | VGB6 | C57BL/6NTac | |
