| Gene | Ttc24 |
| Chromosome | 3 |
| Deletion Start | 88,074,920 |
| Deletion End | 88,069,948 |
| Deletion Size | 4,973 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,074,031 | 88,074,049 | GCATGGCTGGCTGAATCTC |
| TUP | 88,074,001 | 88,074,026 | ACCAGTTACTGGGCTGGGACTACCTG |
| TUR | 88,073,954 | 88,073,973 | CAGGCCACTGTGCTCACTAC |
| TDF | 88,070,388 | 88,070,409 | GCCACAACCCATCTTCCATCTG |
| TDP | 88,070,361 | 88,070,386 | AGGCCCAAGTCCCAGTGGAGAGGAAT |
| TDR | 88,070,310 | 88,070,328 | GGAGGACCCACCTACTGAC |
PCR Primers
| Primer | Sequence |
| SU | CTCAGATTCAGGAGGCCTTG |
| SD | CGGTTATTGTGTGACTTTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12722A-H5 | VGB6 | C57BL/6NTac | |
| 12722A-G2 | VGB6 | C57BL/6NTac | |
| 12722A-F9 | VGB6 | C57BL/6NTac | |
| 12722A-F5 | VGB6 | C57BL/6NTac | |
