12723   Pqlc3     Status: ES cell colonies screened / QC positives

GenePqlc3
Chromosome12
Deletion Start16,999,952
Deletion End16,989,779
Deletion Size10,174
Genome Build38
MGI IDMGI:2444067
NCBI Gene ID217430
BAC ID215G12
BAC LibraryRPCI 23
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 12

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF16,999,05716,999,081GGACTGAATAGAAAGCTCAGGGATG
TUP16,999,03316,999,054TACAGAGTGCCAGCCCAACGTC
TUR16,998,99316,999,011AAAGCCAGGCAGCAAAGAG
TDF16,990,27316,990,294TCAGCCAGGGCTGTATTACTTC
TDP16,990,24616,990,270TGAACCACTCAGGATGCATGGCTGA
TDR16,990,17716,990,199GATTGTGAGAGCATTGGTTCCAG

PCR Primers

PrimerSequence
SUCCCTGGAGTCTGGAAGGAC
SDAACCCAATCTAAGCCCTTCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR89 bp++-
TDFTDR118 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12723E-A11VGB6C57BL/6NTac
12723E-C3VGB6C57BL/6NTac
12723E-C6VGB6C57BL/6NTac
12723E-C9VGB6C57BL/6NTac
12723E-G1VGB6C57BL/6NTac
12723E-H6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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