| Gene | Fam160a1 |
| Chromosome | 3 |
| Deletion Start | 85,722,424 |
| Deletion End | 85,720,419 |
| Deletion Size | 2,006 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,722,273 | 85,722,296 | AAGACCCTTCACTATTCCGATTTC |
| TUP | 85,722,245 | 85,722,263 | AACCCTTTGCTAATTTTGA |
| TUR | 85,722,204 | 85,722,224 | CACGGGATTCAAGCAGAGCAT |
| TDF | 85,720,517 | 85,720,539 | CATAGAAAGTGAGCCCTGTCATC |
| TDP | 85,720,493 | 85,720,516 | TGCTTCTGTCTCAGGTGGCTCACC |
| TDR | 85,720,472 | 85,720,491 | CGAGCTGAGTGCGGATCAAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGCTGTCCACTGCGTTAG |
| SD | ACAGCTTAACAGACACAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12726A-B1 | VGB6 | C57BL/6NTac | |
| 12726A-H7 | VGB6 | C57BL/6NTac | |
