| Gene | Ccdc114 |
| Chromosome | 7 |
| Deletion Start | 45,928,565 |
| Deletion End | 45,948,656 |
| Deletion Size | 20,092 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,928,572 | 45,928,590 | TGGAAAGGCGGGCATACAG |
| TUP | 45,928,591 | 45,928,616 | CAAGGAAGTTCATCAGCGCCTCAGGA |
| TUR | 45,928,651 | 45,928,668 | TCCCAGGGCTCAGAAGTC |
| TDF | 45,948,266 | 45,948,287 | CGAATCCATCCTGAGCCACAAG |
| TDP | 45,948,289 | 45,948,308 | CGAACCGAGGCCGTGGCACT |
| TDR | 45,948,329 | 45,948,350 | AGCAGAGGCAGAGTCACCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GAAGGAAGGGACCATTCTGG |
| SD | TAGGGATGGGCTCACAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12729A-C2 | VGB6 | C57BL/6NTac | |
| 12729B-F2 | VGB6 | C57BL/6NTac | |
