| Gene | Xkr6 |
| Chromosome | 14 |
| Deletion Start | 63,798,283 |
| Deletion End | 63,819,796 |
| Deletion Size | 21,514 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,798,948 | 63,798,966 | TGTGGCCTCGGGTAGTCTC |
| TUP | 63,798,974 | 63,798,998 | CCTCAGAAGCCACAGAGCACTGCCT |
| TUR | 63,799,026 | 63,799,045 | GGCATCCCAGAACCCAAGAG |
| TDF | 63,818,880 | 63,818,898 | ACAAGCTGCTGCGGGACTC |
| TDP | 63,818,899 | 63,818,927 | CAGGGACGACAAGAAGAGCATGAGTTACA |
| TDR | 63,818,936 | 63,818,955 | CGCCAGAAGAGGTGGATGAG |
PCR Primers
| Primer | Sequence |
| SU | ATGCTTATTTGATGGGCCTG |
| SD | CCTAGGAACTGCAGTGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12730A-H8 | VGB6 | C57BL/6NTac | |
| 12730A-D5 | VGB6 | C57BL/6NTac | |
