| Gene | Tdrd6 |
| Chromosome | 17 |
| Deletion Start | 43,630,152 |
| Deletion End | 43,615,823 |
| Deletion Size | 14,330 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,629,242 | 43,629,261 | AGTGCCACCTGGGAGGAGAG |
| TUP | 43,629,217 | 43,629,239 | AGGAGAGCCCTGACAAACCGGGA |
| TUR | 43,629,195 | 43,629,215 | AGCCACAGGAAGCACATGGAG |
| TDF | 43,616,391 | 43,616,412 | ATGAGGAAACCCAGGGCAAGAC |
| TDP | 43,616,362 | 43,616,384 | CAGGAAGCAGAGGCAGGCTCCAT |
| TDR | 43,616,326 | 43,616,349 | AGGACCATAGAGCGAGGTAGTAAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTACTGGATCTCTGCCAG |
| SD | AGACAAACAAGGTTGCTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12734A-B3 | VGB6 | C57BL/6NTac | |
| 12734A-C1 | VGB6 | C57BL/6NTac | |
