| Gene | Plac1l |
| Chromosome | 19 |
| Deletion Start | 11,660,524 |
| Deletion End | 11,647,411 |
| Deletion Size | 13,114 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,660,467 | 11,660,488 | GCTGTCTTGGTTTGGACTTGTG |
| TUP | 11,660,440 | 11,660,464 | TGGGACATTGACGGTAAGTGAGCAC |
| TUR | 11,660,391 | 11,660,412 | CCTGCATGGCAAGCAATCAATC |
| TDF | 11,647,743 | 11,647,765 | CAACGGAGTTACTGACTGGAAAC |
| TDP | 11,647,715 | 11,647,741 | CACTAGGTAGAGCTGGCTTGCACTTGC |
| TDR | 11,647,691 | 11,647,713 | GAAGGCAGAGACAACAGAATCAC |
PCR Primers
| Primer | Sequence |
| SU | GGAGGCATTTAGATGACCTTAAC |
| SD | AAATGTTCAAATTCGCCATTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12736A-B3 | VGB6 | C57BL/6NTac | |
| 12736A-G6 | VGB6 | C57BL/6NTac | |
| 12736A-B5 | VGB6 | C57BL/6NTac | |
