| Gene | Mcart1 |
| Chromosome | 4 |
| Deletion Start | 45,400,134 |
| Deletion End | 45,398,671 |
| Deletion Size | 1,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,399,912 | 45,399,931 | CCTGCAGCTGAGGAAGGATG |
| TUP | 45,399,889 | 45,399,909 | TTCCGCAACTTGTACCGCGGA |
| TUR | 45,399,825 | 45,399,851 | GGTCCTCATACAGACCAAACATAAGTG |
| TDF | 45,398,938 | 45,398,959 | GGCATCTCAGGCTCATTCTCAC |
| TDP | 45,398,904 | 45,398,930 | ACTTTGTCTCTGCTCTTAAGAGGGCCA |
| TDR | | | TGGCACCTCTGCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | GAGTTGAAGGCTATCCTGGG |
| SD | CTGTAGCACTGAGAAACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12738A-A3 | VGB6 | C57BL/6NTac | |
| 12738A-H3 | VGB6 | C57BL/6NTac | |
