| Gene | Gm806 |
| Chromosome | 13 |
| Deletion Start | 50,467,404 |
| Deletion End | 50,475,133 |
| Deletion Size | 7,730 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,467,644 | 50,467,667 | TGCATCAGAAGAAACGACTTAGAC |
| TUP | 50,467,673 | 50,467,697 | CTTCCGGTATGAGAGGCAGGTCTCC |
| TUR | 50,467,726 | 50,467,745 | TCTGGACCACCACAGTGAAC |
| TDF | 50,474,993 | 50,475,012 | GTGTCTGAAGGGCCACTCTC |
| TDP | 50,475,013 | 50,475,037 | AGCTCTGGAGCTGGCTCACCTCTCA |
| TDR | 50,475,079 | 50,475,099 | TGCCTCTTCTTCCTCTTCCTC |
PCR Primers
| Primer | Sequence |
| SU | CGTGAGTTCAGGGTTCTGTG |
| SD | AACTCAAGACCAGAGCAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12739A-B2 | VGB6 | C57BL/6NTac | |
| 12739A-B10 | VGB6 | C57BL/6NTac | |
| 12739A-C5 | VGB6 | C57BL/6NTac | |
| 12739A-C10 | VGB6 | C57BL/6NTac | |
