| Gene | Iqub |
| Chromosome | 6 |
| Deletion Start | 24,505,904 |
| Deletion End | 24,479,151 |
| Deletion Size | 26,754 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,505,775 | 24,505,795 | TGCCAGAGCAAGACGATGAAG |
| TUP | 24,505,752 | 24,505,774 | TGCAGGAACTCACCACCGAGAGC |
| TUR | 24,505,732 | 24,505,751 | TGCCTCTCCACGTTCTCCTC |
| TDF | 24,479,502 | 24,479,526 | CCCATGTTTGATTTCCAGGATTCAC |
| TDP | 24,479,458 | 24,479,487 | AGTCACTGATAGTTTCGGCTCCAGATGAGA |
| TDR | 24,479,439 | 24,479,457 | CCCTTGGAGGCCAGATGTG |
PCR Primers
| Primer | Sequence |
| SU | GAAGGCTGTATGCCTCAGTG |
| SD | TACTTTGTACACTATCAGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12743A-D2 | VGB6 | C57BL/6NTac | |
| 12743A-D3 | VGB6 | C57BL/6NTac | |
| 12743A-E7 | VGB6 | C57BL/6NTac | |
