| Gene | Klhl38 |
| Chromosome | 15 |
| Deletion Start | 58,323,328 |
| Deletion End | 58,314,825 |
| Deletion Size | 8,504 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,322,680 | 58,322,697 | GGAGGCACATGCAGGAAC |
| TUP | 58,322,649 | 58,322,671 | CAGGTCCGCCTGCAGTACATCCA |
| TUR | 58,322,603 | 58,322,622 | CTGGAGGAGGGCGTCATTGG |
| TDF | 58,314,924 | 58,314,943 | GACTGCTACGACCCAGAGAC |
| TDP | 58,314,898 | 58,314,921 | ACACTTGGACATCCCAGGGACAGC |
| TDR | 58,314,868 | 58,314,886 | GGCAGGCATGGTCAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CACTTTGGTTGGAAGATGCC |
| SD | CTGAAGGGTGATTCCAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12746A-B5 | VGB6 | C57BL/6NTac | |
| 12746A-E9 | VGB6 | C57BL/6NTac | |
| 12746A-B4 | VGB6 | C57BL/6NTac | |
| 12746A-G4 | VGB6 | C57BL/6NTac | |
