| Gene | Fam92b |
| Chromosome | 8 |
| Deletion Start | 120,177,330 |
| Deletion End | 120,166,569 |
| Deletion Size | 10,762 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,176,894 | 120,176,913 | TGCTTGCCTGTCTAACTGTG |
| TUP | 120,176,866 | 120,176,892 | ATGAGTGACAGCCATCCTGGAGTCTGG |
| TUR | 120,176,841 | 120,176,859 | ATCCCGCCCAAGGGCATAC |
| TDF | 120,167,196 | 120,167,217 | GACGTGGAGACAGGATGGAGAG |
| TDP | 120,167,173 | 120,167,194 | TCTGGCACCATGGCAGGTCAGA |
| TDR | 120,167,151 | 120,167,172 | CTGTGGCCCTGGTGAAATAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCGTTCCTGGTGGTTACAAG |
| SD | TAGCTGATCGTGACTGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12748A-A5 | VGB6 | C57BL/6NTac | |
| 12748A-A9 | VGB6 | C57BL/6NTac | |
| 12748A-C4 | VGB6 | C57BL/6NTac | |
| 12748A-C7 | VGB6 | C57BL/6NTac | |
| 12748A-F7 | VGB6 | C57BL/6NTac | |
| 12748A-G3 | VGB6 | C57BL/6NTac | |
