| Gene | Zmynd15 |
| Chromosome | 11 |
| Deletion Start | 70,460,573 |
| Deletion End | 70,466,145 |
| Deletion Size | 5,573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,460,734 | 70,460,752 | CCTTTGGGTGCTCCATGTC |
| TUP | 70,460,756 | 70,460,777 | CCAAACCGCAGTGTGGGCATCA |
| TUR | 70,460,838 | 70,460,858 | GGTGAAGTGGAGGCTCATCTC |
| TDF | 70,465,208 | 70,465,229 | TCCTTCCACCACCCTTAAACTG |
| TDP | 70,465,231 | 70,465,257 | TCCGTAGACCTCTCCAGCATTAGGGTC |
| TDR | 70,465,267 | 70,465,284 | ACATCCCGAGACCCATCC |
PCR Primers
| Primer | Sequence |
| SU | GATCCAGACCCAAGAGATGC |
| SD | CAAACCAAGTGTGGATCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12750A-B2 | VGB6 | C57BL/6NTac | |
| 12750A-E11 | VGB6 | C57BL/6NTac | |
| 12750A-F7 | VGB6 | C57BL/6NTac | |
| 12750A-F12 | VGB6 | C57BL/6NTac | |
| 12750A-F4 | VGB6 | C57BL/6NTac | |
| 12750A-H12 | VGB6 | C57BL/6NTac | |
