| Gene | Fam167a |
| Chromosome | 14 |
| Deletion Start | 63,452,259 |
| Deletion End | 63,462,666 |
| Deletion Size | 10,408 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,452,316 | 63,452,334 | GCCGTACCTCCTGATGACC |
| TUP | 63,452,336 | 63,452,359 | CCTCCTCAGTCTGAAGGCCCTCAC |
| TUR | 63,452,361 | 63,452,380 | GTCTCCAGCCTCAGCTTCTC |
| TDF | 63,462,122 | 63,462,142 | CCAGGACTGGCACAGAGAAAG |
| TDP | 63,462,145 | 63,462,170 | TTAGACATAGTCGCTAGCCTGGCACA |
| TDR | 63,462,192 | 63,462,212 | GACTGCGATGGAACGGGATAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTGTCTGATCCTGCCTTC |
| SD | CAGGTTGCTGAGCCTCCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12752A-B5 | VGB6 | C57BL/6NTac | |
| 12752A-B7 | VGB6 | C57BL/6NTac | |
| 12752A-C1 | VGB6 | C57BL/6NTac | |
| 12752A-D4 | VGB6 | C57BL/6NTac | |
