| Gene | Gm5077 |
| Chromosome | 6 |
| Deletion Start | 124,636,028 |
| Deletion End | 124,625,119 |
| Deletion Size | 10,910 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,635,982 | 124,636,006 | AGAAATGAATCCATCTCCACCTTCC |
| TUP | 124,635,937 | 124,635,960 | CACAGGGTGTCACTATGCAGCCCA |
| TUR | 124,635,907 | 124,635,927 | GGACAGAGGAAGGGTGATGTC |
| TDF | 124,625,742 | 124,625,763 | GGCTTTGAGACTGGCTGACTTG |
| TDP | 124,625,713 | 124,625,738 | AATGCCCAGAGGCTCTACACCAAGCG |
| TDR | 124,625,690 | 124,625,711 | TCCAGCCGGGATGAATAATCAC |
PCR Primers
| Primer | Sequence |
| SU | TGTGTGGAAATGCAGAGACC |
| SD | TGTTCCCTGTCTGCATCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12754A-B8 | VGB6 | C57BL/6NTac | |
| 12754A-C1 | VGB6 | C57BL/6NTac | |
| 12754A-F2 | VGB6 | C57BL/6NTac | |
| 12754A-F1 | VGB6 | C57BL/6NTac | |
| 12754A-B2 | VGB6 | C57BL/6NTac | |
| 12754A-G1 | VGB6 | C57BL/6NTac | |
| 12754A-H8 | VGB6 | C57BL/6NTac | |
