12756 Ank1 Status: ES cell colonies screened / QC positives
| Gene | Ank1 |
| Chromosome | 8 |
| Deletion Start | 23,058,635 |
| Deletion End | 23,088,054 |
| Deletion Size | 29,420 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,059,519 | 23,059,542 | CACTGTACTTTAATGCGAGGTCTG |
| TUP | 23,059,551 | 23,059,574 | CCCGGAGGACCAAACCTGGGTGTT |
| TUR | 23,059,589 | 23,059,609 | GTTCCACTCATGCAGCCTAAG |
| TDF | 23,087,355 | 23,087,373 | TTCCTTCCCTGTGCCCTTG |
| TDP | 23,087,374 | 23,087,401 | CTGGAATGAGGAAGTCATCATGGCCAGC |
| TDR | 23,087,406 | 23,087,429 | CCACTTTCTATCTTCCCGCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | AAACAGACTCTGCTGAGCTC |
| SD | GCGACACAGTAGATGGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
