| Gene | Car1 |
| Chromosome | 3 |
| Deletion Start | 14,778,381 |
| Deletion End | 14,766,491 |
| Deletion Size | 11,891 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,777,986 | 14,778,010 | AAGCAAACAGTTAGTTGAAGTCCTG |
| TUP | 14,777,944 | 14,777,970 | CATGCTGTGAAATGGTGGTTGCATGGA |
| TUR | 14,722,424 | 14,722,445 | TGGGATACAGCTTGCTCCATTG |
| TDF | 14,766,989 | 14,767,010 | GCCACTTTACACTGAGCACTAC |
| TDP | 14,766,967 | 14,766,988 | TCCAGGCCCTTTGCATGGGTTA |
| TDR | 14,766,912 | 14,766,936 | TCCTTTAATTCCACATAGCATCTCC |
PCR Primers
| Primer | Sequence |
| SU | TCAGTCACATCTTCCACAAGC |
| SD | AAGCACTAAATCCAGAGACCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 55587 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12757A-B3 | VGB6 | C57BL/6NTac | |
| 12757B-B11 | VGB6 | C57BL/6NTac | |
