| Gene | D1Pas1 |
| Chromosome | 1 |
| Deletion Start | 186,967,879 |
| Deletion End | 186,969,865 |
| Deletion Size | 1,987 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 186,968,044 | 186,968,064 | GGCTCACGGTGGAGTAAAGAC |
| TUP | 186,968,069 | 186,968,092 | ATGCCTACAGCAGCTTCGGGTCCC |
| TUR | 186,968,109 | 186,968,132 | CGGTCACTGAAGAAACTGGACTTG |
| TDF | 186,969,373 | 186,969,396 | GCTGCAAGAGGACTGGATATTTCC |
| TDP | 186,969,399 | 186,969,428 | TGTGAAACATGTCATCAATTTCGACTTGCC |
| TDR | 186,969,456 | 186,969,475 | CACGACCTGTACGGCCAATG |
PCR Primers
| Primer | Sequence |
| SU | ACTCAACCGAGTGCTGTTCC |
| SD | CATTACTGAGCCAACGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12758A-D12 | VGB6 | C57BL/6NTac | |
| 12758A-E9 | VGB6 | C57BL/6NTac | |
| 12758A-F2 | VGB6 | C57BL/6NTac | |
| 12758A-D4 | VGB6 | C57BL/6NTac | |
