| Gene | Sds |
| Chromosome | 5 |
| Deletion Start | 120,479,084 |
| Deletion End | 120,483,854 |
| Deletion Size | 4,771 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,479,211 | 120,479,230 | GCATTGGGCATCTCTGCAAG |
| TUP | 120,479,231 | 120,479,255 | ATGGTACAAGGCAGGGTGGCATCCT |
| TUR | 120,479,286 | 120,479,304 | GCACACCTGCCTCTACAAC |
| TDF | 120,482,898 | 120,482,919 | GCATTTGAGTCAGGCCAGTTTG |
| TDP | 120,482,921 | 120,482,945 | TGCCCTAGACCACTGTGCAGGATCC |
| TDR | 120,482,987 | 120,483,005 | GCATGTGAGAGGTGAGGAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTGTCTGCTGACGTGCTC |
| SD | CCACCCTAGATCCCTGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12764A-C9 | VGB6 | C57BL/6NTac | |
| 12764A-D5 | VGB6 | C57BL/6NTac | |
| 12764A-E8 | VGB6 | C57BL/6NTac | |
| 12764A-F5 | VGB6 | C57BL/6NTac | |
