12769   Retnlb     Status: ES cell colonies screened / QC positives

GeneRetnlb
Chromosome16
Deletion Start48,817,216
Deletion End48,818,809
Deletion Size1,594
Genome Build38
MGI IDMGI:1888505
NCBI Gene ID57263
BAC ID275N7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF48,817,24648,817,267GTCTCCCTTCTCCCACTGATAG
TUP48,817,26948,817,287CCCAGGGAACGCGCAATGC
TUR48,817,31748,817,337TGACGACTGAGAGCTTCCTTG
TDF48,818,56648,818,589AGGGTCTCATCATCTCTTACAGTC
TDP48,818,59048,818,616CAAGGGCTCTGACATCTGTGTCTCACA
TDR48,818,64348,818,662GCCATAGCCACAAGCACATC

PCR Primers

PrimerSequence
SUACTGCTATCCTTGTCCGCTG
SDTTTCAGGCCAACAATACGTTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR92 bp++-
TDFTDR97 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12769A-B5VGB6C57BL/6NTac
12769A-E5VGB6C57BL/6NTac
12769A-G2VGB6C57BL/6NTac
12769A-H9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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