| Gene | Il18bp |
| Chromosome | 7 |
| Deletion Start | 102,017,311 |
| Deletion End | 102,015,739 |
| Deletion Size | 1,573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,016,595 | 102,016,617 | TGTCTCTGTGTTCACCATTCTAC |
| TUP | 102,016,571 | 102,016,594 | TGCCCAGCTTCCCAGATGGTTCCT |
| TUR | 102,016,543 | 102,016,567 | CATTGCCTATGTCTGAGTTCTCTTC |
| TDF | 102,016,057 | 102,016,077 | TGGCCCAGTATCACATCATTC |
| TDP | 102,016,034 | 102,016,056 | TGGCCCAGCTCTGGGTAAGGAAC |
| TDR | 102,015,997 | 102,016,015 | AGTAGCGTTCCTCCCATCC |
PCR Primers
| Primer | Sequence |
| SU | CCTAGAAGCTGACTGCTGCC |
| SD | TGGTCAGAGCTGAGTGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12770A-C8 | VGB6 | C57BL/6NTac | |
| 12770A-F7 | VGB6 | C57BL/6NTac | |
| 12770A-F3 | VGB6 | C57BL/6NTac | |
| 12770A-D7 | VGB6 | C57BL/6NTac | |
