| Gene | Cd248 |
| Chromosome | 19 |
| Deletion Start | 5,068,129 |
| Deletion End | 5,070,486 |
| Deletion Size | 2,358 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,068,656 | 5,068,677 | TGTCTACACCACACCCTTCAAC |
| TUP | 5,068,678 | 5,068,702 | CTGGTTTCCAGCGAGTTCGAATGGC |
| TUR | 5,068,707 | 5,068,725 | AGCTGCCACGGAGCCAAAG |
| TDF | 5,070,182 | 5,070,201 | GCCAAAGCCAAAGGGATGAC |
| TDP | 5,070,204 | 5,070,224 | CTGGCTGCTGGTGGCACTCCT |
| TDR | 5,070,238 | 5,070,256 | GCAGCACCACCAAGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | AACAACGCTGAAGTTTCAGAG |
| SD | GTAAGTAAGGCCCTGCCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12771D-D5 | VGB6 | C57BL/6NTac | |
| 12771D-D8 | VGB6 | C57BL/6NTac | |
| 12771D-E8 | VGB6 | C57BL/6NTac | |
| 12771D-G8 | VGB6 | C57BL/6NTac | |
