| Gene | Mc3r |
| Chromosome | 2 |
| Deletion Start | 172,248,863 |
| Deletion End | 172,249,846 |
| Deletion Size | 984 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 172,248,975 | 172,248,994 | TCATCAAGCCGGAGGTCTTC |
| TUP | 172,248,996 | 172,249,018 | TGGCTCTGGGCATCGTCAGTCTG |
| TUR | 172,249,044 | 172,249,064 | GGTTGCCATTCCTGACCACAG |
| TDF | 172,249,661 | 172,249,680 | TGCCCCACCAATCCCTACTG |
| TDP | 172,249,684 | 172,249,708 | CTGCTACACGGCCCATTTCAACACC |
| TDR | 172,249,718 | 172,249,739 | TGACGGAGTTGCACATGATGAG |
PCR Primers
| Primer | Sequence |
| SU | TGGAGAGAGAGGGTCAGAGC |
| SD | CTTGCCGGAAGTCTAAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12774A-C5 | VGB6 | C57BL/6NTac | |
