| Gene | Tnfsf9 |
| Chromosome | 17 |
| Deletion Start | 57,105,435 |
| Deletion End | 57,107,529 |
| Deletion Size | 2,095 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,105,643 | 57,105,661 | CGCCTGCACTGAACTTCTG |
| TUP | 57,105,666 | 57,105,687 | CGCCACCCAAAGCTCTATGGCC |
| TUR | 57,105,724 | 57,105,744 | TGCGGGTGAAGATAGGAACAC |
| TDF | 57,107,384 | 57,107,405 | TGTGGGTCTGAGGGCTTATCTG |
| TDP | 57,107,408 | 57,107,432 | TGGAGCCCAGGATGCATACAGAGAC |
| TDR | 57,107,467 | 57,107,489 | GTCGGGTTTCACAAGAAAGAGTC |
PCR Primers
| Primer | Sequence |
| SU | CGATATCACATGGTTCCCATC |
| SD | CATAAATGAACACCATACCCATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12779B-C9 | VGB6 | C57BL/6NTac | |
| 12779B-G5 | VGB6 | C57BL/6NTac | |
| 12779B-E1 | VGB6 | C57BL/6NTac | |
| 12779B-H4 | VGB6 | C57BL/6NTac | |
