| Gene | Ifnb1 |
| Chromosome | 4 |
| Deletion Start | 88,522,771 |
| Deletion End | 88,522,192 |
| Deletion Size | 580 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,522,604 | 88,522,622 | CACCTACAGGGCGGACTTC |
| TUP | 88,522,572 | 88,522,599 | TCCCTATGGAGATGACGGAGAAGATGCA |
| TUR | 88,522,545 | 88,522,562 | TGGATGGCAAAGGCAGTG |
| TDF | 88,522,364 | 88,522,383 | TGTCCTCAACTGCTCTCCAC |
| TDP | 88,522,330 | 88,522,359 | AGAGCTATTACTGGAGGGTGCAAAGGTACC |
| TDR | 88,522,298 | 88,522,319 | CCAGGCGTAGCTGTTGTACTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCAGCAATTGGTGAAACTG |
| SD | GCTTTAGAGCCACCTGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12782A-C6 | VGB6 | C57BL/6NTac | |
| 12782A-C11 | VGB6 | C57BL/6NTac | |
| 12782A-D4 | VGB6 | C57BL/6NTac | |
